A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
نویسندگان
چکیده
منابع مشابه
Mechanical ventilation induces neutrophil extracellular trap formation.
BACKGROUND Mechanical ventilation can injure the lung and induce a proinflammatory state; such ventilator-induced lung injury (VILI) is associated with neutrophil influx. Neutrophils release DNA and granular proteins as cytotoxic neutrophil extracellular traps (NETs). The authors hypothesized that NETs were produced in a VILI model and may contribute to injury. METHODS In a two-hit lipopolysa...
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INTRODUCTION Neutrophil extracellular trap (NET) formation was recently identified as a novel mechanism to kill pathogens. However, excessive NET formation in sepsis can injure host tissues. We have recently shown that parenteral vitamin C (VitC) is protective in sepsis. Whether VitC alters NETosis is unknown. METHODS We used Gulo-/- mice as they lack the ability to synthesize VitC. Sepsis wa...
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BACKGROUND The release by neutrophils of DNA-based extracellular traps (NETs) is a recently recognized innate immune phenomenon that contributes significantly to control of bacterial pathogens at tissue foci of infection. NETs have also been implicated in the pathogenesis of non-infectious diseases such as small vessel vasculitis, lupus and cystic fibrosis lung disease. Reactive oxygen species ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2019
ISSN: 1940-087X
DOI: 10.3791/59150